Effect of Piperine on Hypothalamic Pituitary Gonadal Axis of Female Albino Rats: A Randomized Controlled Study

Abstract

Background and Objective: Piperine is a constituent of black pepper. When administered to female adult
albino rats, it might results in increased levels of LH and FSH, and thus causing diminished ability to
procreate. The aim of this study is to determine the deleterious piperine influences on LH and FSH levels of
adult female albino rats.
Method: It is a randomized controlled trial conducted in the department of Anatomy, FPGMI, Lahore. By
random selection from 30 rats, three equal groups were made, cach with 10 animals. Control status was
assigned to group A which was not given any medicine, but only N/S administered with the dosage 10mlkg
body wt./day by using NG tube for a span of 30 days. Group B was given piperine at dosage of Smg/kg/day for
aperiod of thirty days. The C group was administered drug at the dosage of 10mg per kg per day for the same
period of time as A and B via the same route. At completion of thirty days of the study, the animals of the 3
groups were given euthanasia. Dissection was performed, their ovaries were removed, and then fixed in 10%
formalin. 4-5 micrometer sections preparation was done by using the microtome and then H&E staining was
carried out.
Results: FSH and LH levels in serum of group B rats were shown to have increased in comparison to control
group A. The FSH and LH levels in rats of C group were greater than that in rats of Bgroup. When the two
groups were compared, this difference was determined to be extremely of significance having a p-value less
than0.001.
Conclusion: Giving Piperine to female adult albino rats was shown to cause significant increase in levels of
FSHand LH whichis likely to produce negative feedback on hypothalamic-pituitary-gonadal axis and in this
way hamper female fertility during their productive years probably by causing PCOS